cyto_transform is capable of automatically computing transformers and is used to apply a set of transformers to the data.cyto_transformer_combine combines individual transformation definitions into a single list of transformers that can be applied to the data using cyto_transform.cyto_transformer_logicle implements the estimateLogicle version of the logicle transformation.cyto_transformer_biex implements the flowjo_biexp version of the biexponential transformation.cyto_transformer_arcsinh implements the asing_Gml2 version of the arcsinh transformation.cyto_transformer_log implements the flowjo_log_trans version of the log transformation.Exporting FCS files from Attune NxT Software Export the Attune NxT Software data files to be analyzed in FlowJo software using these easy steps: 1. Below is a list of the key functions that you will encounter in this vignette: NxT Software files using FlowJo software version 10.3 and 7.6.5 other versions may require modifications to these instructions, as menus and placement of settings may have changed. For consistency with the RGLab suite of cytometry data analysis packages, CytoExploreR uses a series of wrapper functions to add support for these transformations with improved support for customisation.
In this vignette we will demonstrate how CytoExploreR facilitates fine tuning of transformation parameters and how these optimised transformations can be applied cytometry data. CytoExploreR has full support for log, arcsinh, biexponential and logicle transformations implemented in the flowWorkspace package.
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